Friday, June 26, 2009

What is this? Quick Microbe Meetup!

Stat culture comes in, it is a CSF so you make a gram stain, which looks like this: I know it is easy but what you do think you have? Course of action and what do you expect for culture tommorow!

Result: Upon careful examination of the gram stain you will see Gram negative diplococci that are kidney bean shaped, strongly indicative of the organism Neisseria meningitidis. Neisseria meningitidis usually appears as Gram (-) diplococci that sometimes may display considerable size variation, resist decolorization, and a distinct pink halo around the cells. The physician should be notified immediately so the antibiotic care can be administered.
After 18 to 24 hours the culture should be ready and growth on chocolate agar shows colonies that are generally larger than other gonococcal species, usually attaining a diameter of 1mm or more. Colonies are also low and convex, with a smooth, moist entire edge and a glistening surface. Sheep’s blood may present some alpha hemolysis and are grey, heavily encapsulated strains appear mucoid and are oxidase positive. N.meningitidis can be identified by acid production tests or by chromogeneic enzyme substrate tests. It acidifies glucose and maltose, but not sucrose fructose, or lactose. Serotyping is generally done to obtain epidemiological data on the organism and is done through a simple slide agglutination, also PCR, PFGE, and DNA fingerprinting.
All in all, N.meningitidis is an extremely pathogenic organism that can cause rapid death so stat diagnosis is key.


Anonymous said...

Could you help me if I send you some photographs of cultures. We're (three belgian girls in their last year) trying to identify them for a project of school.
please answer to

Anonymous said...

thank you in advance
betty borgers

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